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Tuesday in Mongolia apr 26 links back home enjoy title h1 columns 4 css th style sheet url background image img prof namsrai jpg link automatic captions num name date time size header a href tz camera correction hours and zone of pictures enter search tags that will be visible on sitemap they not the web page itself professors colton houses near college view ub city gibbons myself you are visitor number to this items was last updated 05 28 02 30 01 mdt share. Wednesday in Mongolia links back home enjoy title h1 columns 4 css th style sheet url background image img jpg link automatic captions num name date time size header a href tz camera correction hours and zone of pictures enter search tags that will be visible on sitemap they not the web page itself you are visitor number to this 71 items was last updated 05 28 02 38 04 mdt share.

Thursday in Mongolia links back home enjoy title h1 columns 4 css th style sheet url background image img jpg link automatic captions num name date time size header a href tz camera correction hours and zone of pictures enter search tags that will be visible on sitemap they not the web page itself prayer wheels gate roof detail door you are visitor number to this 73 items was last updated 05 28 02 37 57 mdt share. Friday in Mongolia and korea links back home enjoy title h1 columns 4 css th style sheet url background image img bathroom jpg link automatic captions num name date time size header a href tz camera correction hours zone of pictures enter search tags that will be visible on sitemap they not the web page itself getting seated before take off from flight monitor driving express lane j willard marriott son our beds you are visitor number to this 19 items was last updated 05 28 02 42 mdt share.

Saturday in Seoul 04 30 links back home enjoy title h1 columns 4 css th style sheet url background image img jpg link automatic captions num name date time size header a href tz camera correction hours and zone of pictures enter search tags that will be visible on sitemap they not the web page itself electricity options don fat mirror skinny origami crocodiles elephant animals you are visitor number to this 89 items was last updated 05 28 02 37 49 mdt share. Family Pictures 05 03 colton links back home on may 3 we got a sizable contingent of the together and decided to shoot some all in there were shots i have pared it down just few best ones enjoy title h1 columns css th style sheet url background image dsc jpg link automatic captions num name date time size header href pix html tz camera correction hours zone enter search tags that will be visible sitemap they not web page itself jump from joseph's lois's dsc top don lois joseph akiko img kids daniel ann ben isaac michael alex you are visitor number this 21 items was last updated 28 02 40 31 mdt share.

My Visit to Washington DC in July back i was for meetings and came a few days early so could sightsee here are some pictures you will notice that tried hard get myself into number of the photographs able frame them because camera canon powershot pro1 has flip out rotatable view finder have reasonably long arms segway adventure old post office bell tower ford's theatre george masonic memorial metrorail tour capitol building monument lincoln general not classified. DC Post Office Bell Tower DC Washington Colton Don Capitol Franklin Lincoln links back home the old has a nice view of lines are lots shorter than for monument enjoy title h1 columns 4 css th style sheet url background image img jpg link automatic captions num name date time size header href tz camera correction in hours and zone pictures enter search tags that will be visible on sitemap they not web page itself lincoln ben front me with wires block window memorial cords ringing bells from street below you visitor number to this 8 items was last updated 05 28 02 27 57 mdt share.

DC Ford's Theatre where Lincoln was Shot 16 jul links back home under the there are exhibits about booth and assassination within president's box has been restored enjoy title h1 columns 3 css th style sheet url background image img jpg link automatic captions num name date time size header table width tr td colspan 4 a href tz camera correction in hours zone of pictures enter search tags that will be visible on sitemap they not web page itself life taller than me skinnier too celebrity pose people milling around other seating front house died you visitor number to this 9 items last updated 05 28 02 27 48 mdt share.

General Pictures of Washington DC 16 jul links back home these are some i took as wandered around might get moved into other collections if a sufficient cluster jumps out at me enjoy title h1 columns 4 css th style sheet url background image img jpg link automatic captions num name date time size header href tz camera correction in hours and zone enter search tags that will be visible on sitemap they not the web page itself justice life conduct state is possible only first it resides hearts souls citizens with smithsonian castle cross walks had count down timers so you knew whether to start or wait national museum american indian wwii memorial pacific side two battles cried view from lincoln arlington cemetery across bridge metrorail stop visitor number this 11 items was last updated 05 28 02 38 12 mdt share.

George Washington Masonic Building the national memorial links back home was a great man freemason said nice things about craft when i saw as went through metrorail station decided should check it out they give free tours and that picture taking allowed so took few to remind me of my visit enjoy title h1 columns 3 css th style sheet url background image img jpg link automatic captions num name date time size header href tz camera correction in hours zone pictures enter search tags will be visible on sitemap not web page itself view from king street inside replica arc covenant holiness lord notice how much looks like bunch 7s knights templar room kinghts observation deck bronze statue with laying cornerstone capitol you are visitor number this 14 items last updated 05 28 02 40 13 mdt share.

DC MetroRail Pictures 16 jul links back home most of these are probably boring shots trains and stations i loved as a kid so find myself attracted to taking even though few people in their right minds would them fascinating do enjoy title h1 columns 4 css th style sheet url background image img jpg link automatic captions num name date time size header href tz camera correction hours zone enter search tags that will be visible on sitemap they not the web page itself king street station tunnel approach 1 8 2 3 5 6 7 approaching train arlington cemetary emerging van dorn platform looking west crossing potomac yellow line is down river george washington rochambeau memorial bridges just up you visitor number this 26 items was last updated 05 28 02 40 07 mdt share.

My Visit to the Washington Monument 16 jul links back home i walked length of capitol mall which is a little over two miles took several pictures but did not get there early enough tickets they distribute free each morning at 8 30 am currently you can also order through internet charge convenience fee seems totally reasonable me only in dc other things are memorials was started before died so it strictly speaking memorial like lincoln's or jefferson's any war enjoy title h1 columns 4 css th style sheet url background image img jpg link automatic captions num name date time size header href br tz camera correction hours and zone enter search tags that will be visible on sitemap web page itself from with your classic photo circled by flags leaning against corner view toward top as seen near lincoln middle arlington bridge walking across visitor number this 10 items last updated 05 28 02 38 20 mdt share.

Eclipse Don Naniloa Laie Moon total lunar hawaii 27 28 aug links back home taken from our front porch resting the camera on railing using swing away view screen instead of eyepiece for increased stability typical shots were about a second long cropped in photoshop 5 0 le to reduce field is canon powershot pro 1 7x optical zoom enjoy title h1 columns 2 css th style sheet url background image jpg link automatic captions time num name date size header href pix html tz correction hours and zone pictures enter search tags that will be visible sitemap they not web page itself don moon 22 06 50 hst you may able make out roofline house at 7 o'clock position starting 9 07 46 cloud ring 08 progression 19 10 here can compare curvature earth's shadow compared how would look half 49 20 24 again note it much wider than 44 25 01 30 09 are visitor number this items was last updated 05 02 38 mdt share.

Train Day Naniloa Laie with thomas and friends links back home labor weekend i decided to haul out my the tank engine sets that got last christmas play grandkids here are a few of pictures we took good time was had by all enjoy title h1 columns 3 css th style sheet url background image img jpg link automatic captions num name date size header href pix html tz camera correction in hours zone enter search tags will be visible on sitemap they not web page itself laie master engineer surveys his first creation mikey watch snake its way through switches joseph surround layout seems more interested road set after nap considerable demolition occurred so created new design shannon introduced features job improves another corner our little world you visitor number this 6 items updated 05 28 02 27 29 mdt share.

Colton P.I.

Wireless Man Beta Don Colton Naniloa Laie porch costume test oct 5 links back home halloween sports a hemispherical mixing bowl lined with deflated winnie the pooh kick ball and held in place by rolls of duct tape atop helmet are genuine magnetically mounted 7 dbi high gain antennas for use front piece which is linksys wrt54g v1 0 router running dd wrt linux tastefully draped around his neck short length cat5 network cable space blanket provides stylish cape if you plug power adapter to entire ensemble actually fully operational wi fi access point photos taken lois enjoy title h1 columns 4 css th style sheet url background image img jpg link automatic captions num name date time size header href pix html tz camera correction hours zone pictures enter search tags that will be visible on sitemap they not web page itself don porch pose 1 2 3 visitor number this 12 items was last updated 05 28 02 41 04 mdt share.

Islet size has also been implicated as predictive, with smaller islets expected to result in better CTO, especially at a marginal IE dose. We do not have a clear explanation for this difference, but it is conceivable that the effect of islet size is marginalized when very high IE doses are transplanted. There are some limitations of this study.

Calculation of the OCR dose is based on the IE dose and thus the values have some inherent uncertainty since they are based on operator-dependent manual counts. In addition, rather than correlating OCR dose only with categorical CTOs, it may also be appropriate to quantify the reduction in exogenous insulin requirements and also account for recipient factors, such as varying insulin sensitivity between individuals. These improvements will aid in the case of islet allotransplant, as we predict that this model will be more difficult to correlate CTO with characterization assays due to confounding factors such as the need for immunosuppression therapy, potential rejection, and the high possibility of multiple preparations being transplanted into a single recipient.

Colton P.I. Protector

Future studies will involve optimizing the predictive capability of OCR dose in the IAT model and examining the utility of these islet product characteristics, specifically OCR dose , on the more challenging case of islet allotransplant. We thank Dr. Julia Goldstein, Dr. Guillermo Arreaza-Rubin and Dr. Thomas Eggermann on helpful discussions on data analysis and presentation. The authors would like to thank the Schulze Diabetes Institute islet isolation team and all of our clinical nurses.

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. National Center for Biotechnology Information , U.

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PLoS One. Published online Aug Klearchos K. Bellin , 2 , 3 David E. Sutherland , 2 , 3 Thomas M. Suszynski , 2 , 3 Jennifer P. Kitzmann , 1 , 2 , 3 Efstathios S. Avgoustiniatos , 2 , 3 Angelika C. Gruessner , 1 Kathryn R. Mueller , 1 , 2 , 3 Gregory J. Beilman , 2 Appakalai N. Balamurugan , 2 , 3 Gopalakrishnan Loganathan , 2 , 3 Clark K.

Colton , 4 Maria Koulmanda , 5 Gordon C. Weir , 6 Josh J. Wilhelm , 2 , 3 Dajun Qian , 7 Joyce C. Niland , 7 and Bernhard J. Hering 2 , 3. Melena D. David E. Thomas M. Jennifer P. Efstathios S. Angelika C. Kathryn R. Gregory J.


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Appakalai N. Clark K. Gordon C. Josh J. Joyce C. Bernhard J. Matthias G von Herrath, Editor. Author information Article notes Copyright and License information Disclaimer. Competing Interests: The authors have declared that no competing interests exist. Received Jul 1; Accepted Jul This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. This article has been cited by other articles in PMC.

Abstract Background Reliable in vitro islet quality assessment assays that can be performed routinely, prospectively, and are able to predict clinical transplant outcomes are needed. Conclusions Commonly used assays to determine whether a clinical islet preparation is of high quality prior to transplantation are greatly lacking in sensitivity and specificity.

Introduction Cell-based transplant therapies may offer a minimally-invasive alternative to solid organ transplants for certain diseases such as diabetes. Open in a separate window. Fig 1.

Metric units of volume review (L and mL) (article) | Khan Academy

Previously published data showing overlap and correlation of islet equivalent dose with clinical transplant outcome. Total pancreatectomy, islet isolation and purification TP-IAT was performed as described previously [ 17 ]. Islet autotransplantation The typical autoislet product preparation time ranged from 3.

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Fig 2. Overlap and correlation of islet characterization methods with clinical transplant outcome. Table 1 Comparison of demographic, isolation, and quality assessment variables between insulin dependent and independent clinical transplant outcome 6—12 months post-transplant. Discussion As islet transplantation moves closer to becoming standard-of-care, in vitro islet quality assessment assays that can be performed routinely and prospectively to predict CTOs are needed.

Acknowledgments We thank Dr. Data Availability All relevant data are within the paper. References 1. Potent induction immunotherapy promotes long-term insulin independence after islet transplantation in type 1 diabetes. Am J Transplant. Improvement in outcomes of clinical islet transplantation: — Diabetes Care. Islet transplantation in type 1 diabetic patients using calcineurin inhibitor-free immunosuppressive protocols based on T-cell adhesion or costimulation blockade.

Experience with a novel efalizumab-based immunosuppressive regimen to facilitate single donor islet cell transplantation. Cellular Transplantation: From Laboratory to Clinic. Waltham, MA: Academic Press; Islet assessment for transplantation. Curr Opin Organ Transplant. Correlation between beta cell mass and glycemic control in type 1 diabetic recipients of islet cell graft. Similar islet function in islet allotransplant and autotransplant recipients, despite lower islet mass in autotransplants.

Islet autotransplant outcomes after total pancreatectomy: a contrast to islet allograft outcomes. The use of the BD oxygen biosensor system to assess isolated human islets of langerhans: oxygen consumption as a potential measure of islet potency.

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Cell Transplant. Glucose-stimulated increment in oxygen consumption rate as a standardized test of human islet quality. Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice. A stirred microchamber for oxygen consumption rate measurements with pancreatic islets.

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